RbAp48 [11G10], Antibody

Legacy ID: 20-272-193321
Size: 0.1 mg
Source: Mouse
Reactivity: Human and Mouse - Not yet tested in other species
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Details for RbAp48 [11G10], Antibody

Clone: 11G10

Isotype: IgG2b

Light chain: kappa

Immunogen: The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.

Antigen Species: Human Positive Control: MOLT4, HeLa, LB22

Target: RbAp48

Localization: Nuclear

Concentration: 1 mg/ml

Purification Note: From hybridoma tissue culture supernatant Storage Buffer: Phosphate-buffered saline, pH 7.4, containing no preservatives

Application Note: For IF: Permeabilization step recommended. Use at 1:500-1:1000 in PBST containing 2%BSA and 10% normal serum at 4ºC overnight. For WB: Use at a dilution of 1:500 -1:2,000 in 5% Milk/PBST at 4ºC overnight. Use under reducing conditions. For IP: se at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.

Target protein molecular weight: 48

Cellular Localization: Nuclear

Expression system: pGEX

Myeloma: NS1 RbAp48-11G10 recognizes full length RbAp48, a 48 kDa nuclear protein first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1.

Function: Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.

Subunit: Interacts with SUV39H1 and HDAC7 (By similarity). Binds directly to helix 1 of the histone fold of histone H4, a region that is not accessible when H4 is in chromatin. Subunit of the chromatin assembly factor 1 (CAF-1) complex, which is composed of RBBP4, CHAF1B and CHAF1A. Subunit of the core histone deacetylase (HDAC) complex, which is composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core HDAC complex associates with SIN3A, ARID4B/SAP180, SAP18, SAP30, SAP130, SDS3/SAP45 and possibly ARID4A/RBP1 and ING1 to form the SIN3 HDAC complex. The core HDAC complex may also associate with MTA2, MBD3, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylase complex (the NuRD complex). The NuRD complex may also interact with MBD3L1 and MBD3L2. Interacts with MTA1. Subunit of the PRC2/EED-EZH2 complex, which is composed of at least EED, EZH2, RBBP4, RBBP7 and SUZ12. The PRC2/EED-EZH2 complex may also associate with HDAC1. Part of the nucleosome remodeling factor (NURF) complex which consists of SMARCA1; BPTF; RBBP4 and RBBP7. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SPEN/MINT. Interacts with BRCA1. Interacts with CREBBP, and this interaction may be enhanced by the binding of phosphorylated CREB1 to CREBBP. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2.

Subcellular Location: Nucleus.

Similarity: Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.

Similarity: Contains 6 WD repeats.

Additional Info for RbAp48 [11G10], Antibody

Name RbAp48 [11G10], Antibody
Related Product Names Mouse monoclonal antibody to human RbAp48; 5928; RBBP4; 602923; Q09028; RBAP48; Rb associated protein 48, Rb-associated protein, RbAp 48RbAp48 [11G10]RbAp48
NCBI Acc Number NP_005601.1
Purity Protein G Affinity Purified
Format Liquid
Clonality Monoclonal
Storage Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.
Molecular Weight 47656
Swiss Prot Number Q09028
Applications IF, IP, WB

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