Assay Development

GenWay specializes in immunoassays since we produce the relevant reagents including proteins and antibodies. We routinely develop and utilize the following assays for specific analysis needs.

An immunoassay is a biochemical test that measures the level of a substance in a biological liquid, typically serum or urine, using the reaction of an antibody or antibodies to its antigen. The assay takes advantage of the specific binding of an antibody to its antigen. Monoclonal antibodies are often used as they only usually bind to one site of a particular molecule, and therefore provide a more specific and accurate test, which is less easily confused by the presence of other molecules. Polyclonal antibodies have also been successfully applied for various immunoassays. The antibodies picked must have a high affinity for the antigen (if there is antigen available, a very high proportion of it must bind to the antibody).

Based upon the capability and core competency in protein and antibody, we have been providing assay development and standardization for our clients.

For more detailed service items, please see the following pages Service Description:

• ELISA
  
  • Direct ELISA
  • Sandwich ELISA

GenWay also provides technology description and specific resources on immunoassays:

Technologies – Immunoassays

• ELISA
  
  • Direct ELISA
  • Sandwich ELISA
  • Competitive ELISA

Technologies – Multiplex Applications

GenWay's technology platform enables us in developing various applications of antibody and protein arrays

• Antibody and Protein Arrays

Immunoassay Wikipedia

1.0 Overview of Immunoassay

• 1.1 Introduction of immunoassay
• 1.2 Features of various assays
• 1.3 Comparison of immunoassays

2.0 Western Blot

• 2.1 Description
• 2.2 Methods
• 2.3 Application

3.0 ELISA

• 3.1 Direct ELISA
• 3.2 Indirect ELISA
• 3.3 Sandwich ELISA
• 3.4 Competitive ELISA
• 3.5 Multiplex ELISA

4.0 ELISpot Assay

• 4.1 Description
• 4.2 Application

5.0 Flow Cytometry (FLOW)

• 5.1 Description
• 5.2 Sample preparation
• 5.3 Application

6.0 Immunoprecipitation (IP)

• 6.1 Description
• 6.2 Protein-Protein IP
• 6.3 Protein-DNA (Chromatin) IP
• 6.4 Application

7.0 Immunocytochemistry (ICC)

• 7.1 Description
• 7.2 Co-Staining of IgY with IgG
• 7.3 Application

8.0 Immunohistochemistry (IHC)

• 8.1 Description
• 8.2 Methods
• 8.3 IgY Usages in IHC
• 8.4 Application

9.0 Summary