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Protein Expression

Protein Expression

Protein expression is an art of science and technology. Each protein is different and needs special treatment. We provide customized solutions to each protein expression project. GenWay has developed a multi-tiered platform of protein expression. This system, consisting of different vector and host systems, can be applied for expression and purification of difficult proteins such membrane proteins, kinases, proteases, nuclear receptors, etc. The key features of our technologies are to produce proteins, protein fragments, or fusion proteins that are soluble, functional, and applicable for various uses. GenWay's team has produced more than 2,500 recombinant proteins. Our proprietary technology, know-how, and experience enable us to provide most capable and powerful protein production services to our clients.

GenWay's facility:

5,000 sq ft Laboratory space
cGMP compliant for Diagnostics
Class 10,000 clean room

For more detailed service items, please see the following Service Description pages:

GenWay also provides technology description and specific resources on protein production:

Technologies – Protein Expression

E. coli Expression Technologies
Yeast Expression Technologies
Mammalian Cell Expression Technologies
- Stable Cell Line
- Transient Cells
- Viral-Vector Driven

Technologies – Antigen Preparation

Quality antigens prime quality antibodies. GenWay specializes in high-quality antigens...More

Native Proteins
Peptides and Conjugates
Recombinant Proteins
- Full-Length
- Domain-Specific
- Fusion or Tagged

Protein Expression

1.0 Overview of Protein Expression

1.1 Protein Expression Systems
1.2 GenWay's Multi tiered platform
1.3 Comparison of expression systems
1.4 Bioinformatics and structure analyses

2.0 E. coli Expression System

2.1 Initial expression screening
2.2 Optimization of expression levels
2.3 Improving protein solubility
2.4 Improving protein stability
2.5 Decreasing protein toxicity
2.6 In vitro expression using E. coli extracts
2.7 Co-expression
2.8 Functional protein expression example

3.0 Yeast Expression System

3.1 Superior expression
3.2 High cell densities
3.3 Controllable process
3.4 Mammalian-like proteins
3.5 Generations of stability
3.6 Durability
3.7 Maximum Value
3.8 Expression example

4.0 Mammalian Expression System

4.1 Usage of Mammalian Expression
4.2 Overview of mammalian expression
4.3 Transgene sequence optimization
4.4 Membrane protein expression
4.5 Production of fusion proteins
4.6 GenWay's specialty

5.0 Summary

1.0 Overview of Protein Expression
1.1 Protein expression systems 1.2 GenWay's multi-tiered platform 1.3 Comparison of expression systems 1.4 Bioinformatics and structure analyses
2.0 E. coli Expression System
2.1 Initial expression screening 2.2 Optimization of expression levels 2.3 Improving protein solubility 2.4 Improving protein stability 2.5 Decreasing protein toxicity 2.6 In vitro expression using E. coli extracts 2.7 Co-expression 2.8 Functional protein expression example
3.0 Yeast Expression System
3.1 Superior expression 3.2 High cell densities 3.3 Controllable process 3.4 Mammalian-like proteins 3.5 Generations of stability 3.6 Durability 3.7 Maximum Value 3.8 Expression example
4.0 Mammalian Expression System
4.1 Usage of Mammalian Expression 4.2 Overview of mammalian expression 4.3 Transgene sequence optimization 4.4 Membrane protein expression 4.5 Production of fusion proteins 4.6 GenWay's specialty
5.0 Summary