Antigen Preparation
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Antigen Preparation
One of the determining factors in quality antibody development is quality antigen preparation. We focus on preparation of high-quality antigen for ensuring high-quality antibody development.
- Using our bioinformatics core we determine the best immunogenic domain for antibody generation.
- Using our proprietary fusion domain and vectors we express and purify recombinant antigens in the most cost-effective manner.
- GenWay can provide full services, with the customer providing a target protein accession number and GenWay delivering high-quality polyclonal or monoclonal antibodies.
There are different natures and great variations of antigens in type, form, size, availability, etc. Each type of antigen has its own utility, advantages and disadvantages. Table 1 provides an overview and comparison of antigen considerations.
| Comparison of Antigens | ||||
| Consideration | Native Proteins | Recombinant Proteins | Peptides | Others |
| Size and form | Full-Length Vary in size |
50-300 aa (Domain) or Full-Length |
6-20 aa | Small molecule, macromolecule whole cell or microorganism |
| Conjugation (Carrier) | Not Needed | Not Needed | Needed | Needed for small and macromolecule |
| Fusion Tag | No | Optional | No | Optional |
| Immunogenicity | Best | High | Fair | Fair |
| Success Rate for Antibody Generation |
80-95% | 60-80% | 30-60% | 40-60% |
| Suitable as Ligand | Best | Good | Fair | Vary |
| Immuno-reactivity | Good | Good | Vary | Vary |
| Reproducibility | Best | Good | Good | Vary |
| Cost | High | Middle | Low | Vary |
GenWay's antigen technology specializes in the following three major types:
GenWay offers custom services in antigen or antibody development. For more detailed information,
please see the specific description pages in protein expression and custom antibodies.