Thyroid Stimulating Hormone (TSH) Enzyme Immunoassay Test Kit

GenWay ID: GWB-65C26B
Legacy ID: 40-052-115002
Size: ELISA_Kits
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Details for Thyroid Stimulating Hormone (TSH) Enzyme Immunoassay Test Kit

Species Human.
Sample Type Serum. For the quantitative determination of thyroid stimulating hormone (TSH) concentration in human serum. Blood, Endocrine Glands, hyroid Diseases, Central Nervous System, Neurosecretory Systems Please click here for MSDS PDF Datasheet.
Product Name Thyroid Stimulating Hormone (S-TSH).
Sample Type Serum.
# of Samples 1X96 Assays.
Intended Use For the quantitative determination of thyroid stimulating hormone (TSH) concentration in human serum. The assay is useful in the diagnosis of thyroid or pituitary disorders.
Introduction The determination of serum or plasma levels of thyroid stimulating hormone (TSH or thyrotropin) is recognized as an important measurement in the assessment of thyroid function.
  1. Thyroid stimulating hormone is secreted by the anterior lobe of the pituitary gland, and induces the production and release of thyroxine (T4) and triiodothyronine (T3) from the thyroid gland.
  2. It is a glycoprotein with a molecular weight of approximately 28,000 daltons, consisting of two chemically different subunits, alpha and beta.
  3. Although the concentration of TSH in the blood is extremely low, it is essential in the maintenance of normal thyroid function. The release of TSH is regulated by a TSH-releasing hormone (TRH) produced by the hypothalamus. The levels of TSH and TRH are inversely related to the level of thyroid hormone. When there is a high level of thyroid hormone in the blood, less TRH is released by the hypothalamus, so less TSH is secreted by the pituitary. The opposite action will occur when there are decreased levels of thyroid hormones in the blood. This process, known as a negative feedback mechanism, is responsible for maintaining the proper blood levels of these hormones.
  4. The GenWay Biotech S-TSH offers a sensitivity of 0.05 ?IU/mL and hence allows discrimination between hyperthyroid and normal populations. The assay is intended to quantitatively measure TSH in human serum with 2nd generation sensitivity.
Principles of the Assay The GenWay Biotech Sensitive-TSH ELISA Test is based on the principle of a solid phase enzyme-linked immunosorbent assay.^6,7 The assay system utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the intact TSH molecule. Mouse monoclonal anti-TSH antibody is used fore solid phase (microtiter wells) immobilization, and goat anti-TSH antibody is used in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react simultaneously with the antibodies, resulting in the TSH molecule being sandwiched between the solid phase and enzyme-linked antibodies. After a 2 hour incubation at room temperature with shaking, the solid phase is washed with distilled water to remove unbound labeled antibodies. A solution of tetramethylbenzidine (TMB) is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of 1N HCl, and the resulting yellow color is measured spectrophotometrically at 450 nm. The concentration of TSH is directly proportional to the color intensity of the test sample.
Storage and Stability 1. Store the unopened kit at 2°C-8°C upon receipt and when it is not in use, until the expiration shown on the kit label. Refer to the package label for the expiration date.
2. Keep microtiter plate in a sealed bag with desiccant to minimize exposure to damp air.
Limitation of the Test 1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the package insert instructions and with adherence to good laboratory practice.
2. Serum samples demonstrating gross lipemia, gross hemolysis, or turbidity should not be used with this test.
3. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings.
4. The GenWay Biotech S-TSH assay has not been tested on newborns, and is not for use in screening newborns.
Subunit Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin.
Subcellular Location Secreted.
Similarity Belongs to the glycoprotein hormones subunit alpha family.

Additional Info for Thyroid Stimulating Hormone (TSH) Enzyme Immunoassay Test Kit

Name Thyroid Stimulating Hormone (TSH) Enzyme Immunoassay Test Kit
Related Product Names HTSHR-I; Thyroid Stimulating Hormone (S-TSH) ELISA Kit; Anterior pituitary glycoprotein hormones common subunit alpha; Follitropin alpha chain; Follicle-stimulating hormone alpha chain; FSH-alpha; Lutropin alpha chain; Luteinizing hormone alpha chain; LSH
NCBI Acc Number NP_000726.1
Molecular Weight 13075
Swiss Prot Number P01215

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