Details for Thyroid Stimulating Hormone (TSH) Enzyme Immunoassay Test Kit
|Sample Type||Serum. For the quantitative determination of thyroid stimulating hormone (TSH) concentration in human serum. Blood, Endocrine Glands, hyroid Diseases, Central Nervous System, Neurosecretory Systems Please click here for MSDS PDF Datasheet.|
|Product Name||Thyroid Stimulating Hormone (S-TSH).|
|# of Samples||1X96 Assays.|
|Intended Use||For the quantitative determination of thyroid stimulating hormone (TSH) concentration in human serum. The assay is useful in the diagnosis of thyroid or pituitary disorders.|
|Introduction||The determination of serum or plasma levels of thyroid stimulating hormone (TSH or thyrotropin) is recognized as an important measurement in the assessment of thyroid function.
|Principles of the Assay||The GenWay Biotech Sensitive-TSH ELISA Test is based on the principle of a solid phase enzyme-linked immunosorbent assay.^6,7 The assay system utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the intact TSH molecule. Mouse monoclonal anti-TSH antibody is used fore solid phase (microtiter wells) immobilization, and goat anti-TSH antibody is used in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react simultaneously with the antibodies, resulting in the TSH molecule being sandwiched between the solid phase and enzyme-linked antibodies. After a 2 hour incubation at room temperature with shaking, the solid phase is washed with distilled water to remove unbound labeled antibodies. A solution of tetramethylbenzidine (TMB) is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of 1N HCl, and the resulting yellow color is measured spectrophotometrically at 450 nm. The concentration of TSH is directly proportional to the color intensity of the test sample.|
|Storage and Stability||1. Store the unopened kit at 2°C-8°C upon receipt and when it is not in use, until the expiration shown on the kit label. Refer to the package label for the expiration date.
2. Keep microtiter plate in a sealed bag with desiccant to minimize exposure to damp air.
|Limitation of the Test||1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the package insert instructions and with adherence to good laboratory practice.
2. Serum samples demonstrating gross lipemia, gross hemolysis, or turbidity should not be used with this test.
3. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings.
4. The GenWay Biotech S-TSH assay has not been tested on newborns, and is not for use in screening newborns.
|Subunit||Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin.|
|Similarity||Belongs to the glycoprotein hormones subunit alpha family.|
Additional Info for Thyroid Stimulating Hormone (TSH) Enzyme Immunoassay Test Kit
|Name||Thyroid Stimulating Hormone (TSH) Enzyme Immunoassay Test Kit|
|Related Product Names||HTSHR-I; Thyroid Stimulating Hormone (S-TSH) ELISA Kit; Anterior pituitary glycoprotein hormones common subunit alpha; Follitropin alpha chain; Follicle-stimulating hormone alpha chain; FSH-alpha; Lutropin alpha chain; Luteinizing hormone alpha chain; LSH|
|NCBI Acc Number||NP_000726.1|
|Swiss Prot Number||P01215|