Anti- 14-3-3 zeta (Ab-58) Antibody (GWB-9608D8)



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Binding Site: around Ser58 WB Dilution: 500~1000

IHC Dilution: 50~100 Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Subunit: Homodimer. Interacts with PCTK1. Binds to the C-terminal part of WEE1. Interacts with BSPRY. Binds to phosphorylated MLF1 and retains it in the cytoplasm (By similarity). Interacts with MLLT7/FOXO4 and SSH1.

Subcellular Location: Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Ptm: Isoform delta differs from isoform zeta in being phosphorylated (By similarity).

Similarity: Belongs to the 14-3-3 family. [1] Zupan L.A., Steffens D.L., Berry C.A., Landt M.L., Gross R.W.
Cloning and expression of a human 14-3-3 protein mediating phospholipolysis. Identification of an arachidonoyl-enzyme intermediate during catalysis.
[2] Seluja G.A., Pietromonaco S.F., Elias L.
Two unique 5\' untranslated regions in mRNAs encoding human 14-3-3 zeta: differential expression in hemopoietic cells.
[3] Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.
Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.
[4] Bienvenut W.V., Potts A., Barblan J., Claeys D., Quadroni M.
Unpublished observations (NOV-2005).
[5] Lubec G., Afjehi-Sadat L.
Submitted (MAR-2007) to UniProtKB.
[6] Nagata-Ohashi K., Ohta Y., Goto K., Chiba S., Mori R., Nishita M., Ohashi K., Kousaka K., Iwamatsu A., Niwa R., et al.
A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia.
[7] Obsilova V., Vecer J., Herman P., Pabianova A., Sulc M., Teisinger J., Boura E., Obsil T.
14-3-3 protein interacts with nuclear localization sequence of forkhead transcription factor FoxO4.
[8] Soosairajah J., Maiti S., Wiggan O., Sarmiere P., Moussi N., Sarcevic B., Sampath R., Bamburg J.R., Bernard O.
Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin.
[9] Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
[10] Chi A., Valencia J.C., Hu Z.-Z., Watabe H., Yamaguchi H., Mangini N.J., Huang H., Canfield V.A., Cheng K.C., Yang F., et al.
Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.

Additional Information

Name Anti- 14-3-3 zeta (Ab-58) Antibody (GWB-9608D8)
Related Product Names Protein kinase C inhibitor protein 1; KCIP-1 14-3-3 zeta (Ab-58)14-3-3 zeta
Swissprot ID P63104
Molecular Weight 27745
Purity Affinity Purified
Source Rabbit
Application IHC, WB
Clonality Polyclonal
Format In PBS, 0.02% sodium azide and 50% glycerol.
Stability 1 year at -20C
Reactivity Human, Mouse, Rat
Storage 4C for short term (weeks) and -20C for long term. Avoid frequent freeze and thaw.
Datasheets/Manuals Printable datasheet for GWB-9608D8
Intended Use Research Use Only