ABCC11 ELISA Kit (Human) (GWB-KBBVO4)

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GWB-KBBVO4

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Additional Information

Name ABCC11 ELISA Kit (Human) (GWB-KBBVO4)
Description of Target Participates in physiological processes involving bile acids, conjugated steroids and cyclic nucleotides. Enhances the cellular extrusion of cAMP and cGMP. Stimulates the ATP-dependent uptake of a range of physiological and synthetic lipophilic anions, in
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.103 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ABCC11
Gene id 85320
Alias Symbols ATP-binding cassette sub-family C member 11, EWWD, MRP8, Multidrug resistance-associated protein 8, WW
Swissprot ID Q96J66
Protein Name ATP-binding cassette sub-family C member 11
Kit Principle Genway Biotech ABCC11 ELISA Kit (Human) (GWB-KBBVO4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABCC11 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ABCC11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABCC11 captured in the well.
Kit Component
ComponentAmount
Anti-Rgs6 Microplate96 Wells (12 x 8 Well Strips)
Rgs6 Lyophilized Standard2 x 20 ng
100X Biotinylated Rgs6 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_115972
Nucleotide Accession Num NM_032583
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBVO4
Species Reactivity Human