ABCC5 ELISA Kit (Human) (GWB-KBBEC8)

GWB-KBBEC8

96 Wells
$605.00
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Additional Information

Name ABCC5 ELISA Kit (Human) (GWB-KBBEC8)
Availability In Stock
Kit Range 0.156-10 ng/mL
Sensitivity < 0.051 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol ABCC5
Gene id 10057
Alias Symbols ABC33, ATP-binding cassette sub-family C member 5, DKFZp686C1782, EST277145, MOATC, MOAT-C, MRP5, Multidrug resistance-associated protein 5, Multi-specific organic anion transporter C, pABC11, SMRP
Swissprot ID O15440
Protein Name multidrug resistance-associated protein 5
Description of Target The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, M
Kit Principle Genway Biotech ABCC5 ELISA Kit (Human) (GWB-KBBEC8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABCC5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ABCC5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABCC5 captured in the well.
Kit Component
ComponentAmount
Anti-ABCC5 Microplate96 Wells (12 x 8 Well Strips)
ABCC5 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ABCC5 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001018881.1
Nucleotide Accession Num NM_001023587.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBEC8
Species Reactivity Human