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96 Wells
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Additional Information

Name ABI1 ELISA Kit (Human) (GWB-KBBIN2)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.055 ng/mL
Sample Type Serum, plasma
Kit Detection Absorbance, OD 450 nm
Gene Symbol ABI1
Gene id 10006
Alias Symbols Abelson interactor 1, Abi-1, ABI-1, Abl-binding protein 4, AblBP4, Abl interactor 1, e3B1, E3B1, Eps8-binding protein, Eps8 SH3 domain-binding protein, Nap1-binding protein, Nap1BP, NAP1BP, Spectrin SH3 domain-binding protein 1, SSH3BP, SSH3BP1
Swissprot ID Q8IZP0
Protein Name Abl interactor 1
Description of Target This gene encodes a member of the Abelson-interactor family of adaptor proteins. These proteins facilitate signal transduction as components of several multiprotein complexes, and regulate actin polymerization and cytoskeletal remodeling through interacti
Kit Principle Genway Biotech ABI1 ELISA Kit (Human) (GWB-KBBIN2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABI1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ABI1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABI1 captured in the well.
Kit Component
Anti-ABI1 Microplate96 Wells (12 x 8 Well Strips)
ABI1 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ABI1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001012768.1
Nucleotide Accession Num NM_001012750.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBIN2
Species Reactivity Human