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ABI1 ELISA Kit (Human) (GWB-KBBIP5)

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GWB-KBBIP5

96 Wells
$659.00
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Additional Information

Name ABI1 ELISA Kit (Human) (GWB-KBBIP5)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.055 ng/mL
Sample Type serum, plasma and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ABI1
Gene id 10006
Alias Symbols Abelson interactor 1, Abi-1, ABI-1, Abl-binding protein 4, AblBP4, Abl interactor 1, e3B1, E3B1, Eps8-binding protein, Eps8 SH3 domain-binding protein, Nap1-binding protein, Nap1BP, NAP1BP, Spectrin SH3 domain-binding protein 1, SSH3BP, SSH3BP1
Swissprot ID Q8IZP0
Protein Name abl interactor 1
Description of Target May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signa
Kit Principle Genway Biotech ABI1 ELISA Kit (Human) (GWB-KBBIP5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABI1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ABI1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABI1 captured in the well.
Kit Component
ComponentAmount
Anti-NUDC Microplate96 Wells (12 x 8 Well Strips)
NUDC Lyophilized Standard2 x 10 ng
100X Biotinylated NUDC Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001012768
Nucleotide Accession Num NM_001012750
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)96-105%86-93%84-99%95-103%
EDTA Plasma (n=5)79-89%78-94%92-99%94-101%
Heparin Plasma (n=5)78-104%84-98%81-96%79-105%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)90-10495
EDTA Plasma (n=5)94-10599
Heparin Plasma (n=5)87-10194
Datasheets/Manuals Printable datasheet for GWB-KBBIP5
Species Reactivity Human