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Hsp90a Assay kit

GWB-1A8EA8

384reactions
$1,065.90
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Description

Description: The Hsp90alpha Assay Kit is designed for identification of Hsp90alpha inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin for binding to purified recombinant Hsp90alpha. Hsp90alpha is a molecular chaperone with essential functions in maintaining transformation. Inhibition of Hsp90alpha functions has been shown to play a role in tumorigenesis and disease progression. The Hsp90alpha inhibitor screening assay kit comes in a convenient 384-well format, with purified Hsp90alpha enzyme, fluorescently labeled geldanamycin and Hsp90alpha assay buffer for 400 enzyme reactions. The key to the Hsp90alpha Assay Kit is the fluorescently labeled geldanamycin. Using this kit, only one simple step on a microtiter plate is required for Hsp90alpha reactions. The fluorescently labeled geldanamycin is incubated with a sample containing Hsp90alpha enzyme to produce a change in fluorescent polarization that can then be measured using a fluorescence reader. COMPONENTS: HSP90a recombinant enzyme, FITC-labeled geldanamycin, 5X assay buffer, black microtiter plate. APPLICATIONS: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. STABILITY: 1 year when stored as listed. REFERENCES: 1. Kim J, Felts S, LLauger L, He H, Huezo H, Rosen N, and Chiosis G. J Biomol Screening 2004; 9(5): 375-381. 2. Howes R, Barril X, Dymock BW, Grant K, Northfield CJ, Robertson AGS, Surgenor A, Wayne J, Wright L, James K, Matthews T, Cheung KM, McDonald E, Workman P, Drysdale MJ. Anal Biochem 2006; 350:202-213.

Format: assay kit

Scientific Category: Misc. Heat Shock Proteins

Additional Information

Name Hsp90a Assay kit
Related Product Names Hsp90a Assay kit
Datasheets / Downloads GWB-1A8EA8 Datasheet
Purity NA
Format Assay Kit
Storage Mixed storage
Intended Use Research Use Only