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PDE11A4, Active Human Recombinant Protein

GWB-11830F

10µg
$510.00
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Description

Description: Human PDE11A4 (GenBank Accession No. BAB62712), full length with N-terminal GST tag, MW= 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Source: Baculovirus infected Sf9 cells Formulated in: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 5 mM glutathione, and 3 mM DTT. Stability: >6 months at -80°C Purity: >80% by SDS-PAGE . Specific Activity: 2008 U/ug. 1 unit is defined as the amount of enzyme that will convert 1 pmole of 3?, 5?-cGMP to 5? GMP per min at 30 °C in a reaction buffer of 10 mM Tris-HCl, pH 7.4, 0.2 μM MgCl2. Assay buffer: 10 mM Tris-HCl, pH 7.4, 1.0 mM MgCl2 , 0.4 mM 3?, 5?- cGMP substrate, 1 ng/μl PDE11A4. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference: 1. Loughney K, Taylor J, and Florio VA Int J Impot Res. 2005, 17(4):320-5 2. Weeks JL, Zoraghi R, Beasley A, Sekhar KR, Francis SH, Corbin JD. Int J Impot Res. 2005 Jan-Feb;17(1):5-9

Host: baculovirus

Tag: N-terminal GST tag

GI Num: BAB62712

MW: 131 kDa

Format: aqueous buffer solution

Formula: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

Scientific Category: PDE

Species: human

Additional Information

Name PDE11A4, Active Human Recombinant Protein
Related Product Names PDE, phosphodiesterase
Molecular Weight 131 kDa
Datasheets / Downloads GWB-11830F Datasheet
Purity 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Source baculovirus
GI Number BAB62712
Format Aqueous Buffer Solution
Reactivity Human
Storage -80°C
Intended Use Research Use Only