ACTN2 ELISA Kit (Human) (GWB-KBBZT5) (Aviva Cat. No. OKCD01508)

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GWB-KBBZT5

Aviva Cat. No.: OKCD01508

Additional Information

Name ACTN2 ELISA Kit (Human) (GWB-KBBZT5)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.122 ng/mL
Sample Type tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ACTN2
Gene id 88
Alias Symbols Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein
Swissprot ID P35609
Protein Name Alpha-actinin-2
Description of Target F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Kit Principle Genway Biotech ACTN2 ELISA Kit (Human) (GWB-KBBZT5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACTN2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACTN2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACTN2 captured in the well.
Kit Component
ComponentAmount
Anti-ACTN2 Microplate96 Wells (12 x 8 Well Strips)
ACTN2 Lyophilized Standard2 x 20 ng
100X Biotinylated ACTN2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001094
Nucleotide Accession Num NM_001103
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBZT5
Species Reactivity Human