ACVR1 ELISA Kit (Human) (GWB-KBBHH2)

GWB-KBBHH2

96 Wells
$520.00
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Additional Information

Name ACVR1 ELISA Kit (Human) (GWB-KBBHH2)
Description of Target On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.063 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ACVR1
Gene id 90
Alias Symbols Activin receptor-like kinase 2, Activin receptor type-1, Activin receptor type I, ACTRI, ACTR-I, ACVR1A, ACVRLK2, ALK2, ALK-2, FOP, Serine/threonine-protein kinase receptor R1, SKR1, TGF-B superfamily receptor type I, TSRI, TSR-I
Swissprot ID Q04771
Protein Name activin receptor type-1
Kit Principle Genway Biotech ACVR1 ELISA Kit (Human) (GWB-KBBHH2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACVR1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACVR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACVR1 captured in the well.
Kit Component
ComponentAmount
Anti-BLG Microplate96 Wells (12 x 8 Well Strips)
BLG Lyophilized Standard2 x 10 ng
100X Biotinylated BLG Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001096
Nucleotide Accession Num NM_001105
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBHH2
Species Reactivity Human