ACVR1 ELISA Kit (Human) (GWB-KBBMD2)

GWB-KBBMD2

96 Wells
$560.00
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Additional Information

Name ACVR1 ELISA Kit (Human) (GWB-KBBMD2)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.063 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol ACVR1
Gene id 90
Alias Symbols Activin receptor-like kinase 2, Activin receptor type-1, Activin receptor type I, ACTRI, ACTR-I, ACVR1A, ACVRLK2, ALK2, ALK-2, FOP, Serine/threonine-protein kinase receptor R1, SKR1, TGF-B superfamily receptor type I, TSRI, TSR-I
Swissprot ID Q04771
Protein Name Activin receptor type-1
Kit Principle Genway Biotech ACVR1 ELISA Kit (Human) (GWB-KBBMD2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACVR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACVR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACVR1 captured in the well.
Kit Component
ComponentAmount
Anti-ACVR1 Microplate96 Wells (12 x 8 Well Strips)
ACVR1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ACVR1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001096.1
Nucleotide Accession Num NM_001105.4
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBMD2
Species Reactivity Human