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96 Wells

Additional Information

Name ADAM15 ELISA Kit (Human) (GWB-KBBNK1)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.054 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol ADAM15
Gene id 8751
Alias Symbols ADAM 15, Disintegrin and metalloproteinase domain-containing protein 15, MDC15, MDC-15, Metalloprotease RGD disintegrin protein, Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15, Metargidin
Swissprot ID Q13444
Protein Name Disintegrin and metalloproteinase domain-containing protein 15
Description of Target The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytoki
Kit Principle Genway Biotech ADAM15 ELISA Kit (Human) (GWB-KBBNK1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAM15 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAM15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAM15 captured in the well.
Kit Component
Anti-ADAM15 Microplate96 Wells (12 x 8 Well Strips)
ADAM15 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ADAM15 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001248393.1
Nucleotide Accession Num NM_001261464.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBNK1
Species Reactivity Human