ADAM15 ELISA Kit (Human) (GWB-KBBPI7)

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GWB-KBBPI7

96 Wells
$659.00
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Additional Information

Name ADAM15 ELISA Kit (Human) (GWB-KBBPI7)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.054 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ADAM15
Gene id 8751
Alias Symbols MDC15, Metargidin, Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15, Metalloprotease RGD disintegrin protein
Swissprot ID Q13444
Protein Name disintegrin and metalloproteinase domain-containing protein 15
Description of Target The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytoki
Kit Principle Genway Biotech ADAM15 ELISA Kit (Human) (GWB-KBBPI7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAM15 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAM15 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAM15 captured in the well.
Kit Component
ComponentAmount
Anti-PIK3CB Microplate96 Wells (12 x 8 Well Strips)
PIK3CB Lyophilized Standard2 x 10 ng
100X Biotinylated PIK3CB Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001248393
Nucleotide Accession Num NM_001261464
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)96-103%87-96%79-95%97-104%
EDTA Plasma (n=5)79-96%83-95%84-92%95-103%
Heparin Plasma (n=5)92-102%87-102%80-90%79-99%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)86-10192
EDTA Plasma (n=5)78-9187
Heparin Plasma (n=5)92-10195
Datasheets/Manuals Printable datasheet for GWB-KBBPI7
Species Reactivity Human