ADAM19 ELISA Kit (Human) (GWB-KBBDX1)

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GWB-KBBDX1

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Additional Information

Name ADAM19 ELISA Kit (Human) (GWB-KBBDX1)
Description of Target Participates in the proteolytic processing of beta-type neuregulin isoforms which are involved in neurogenesis and synaptogenesis, suggesting a regulatory role in glial cell. Also cleaves alpha-2 macroglobulin. May be involved in osteoblast differentiatio
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.108 ng/mL
Sample Type tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ADAM19
Gene id 8728
Alias Symbols ADAM 19, Disintegrin and metalloproteinase domain-containing protein 19, FKSG34, MADDAM, Meltrin-beta, Metalloprotease and disintegrin dendritic antigen marker, MLTNB
Swissprot ID Q9H013
Protein Name Disintegrin and metalloproteinase domain-containing protein 19
Kit Principle Genway Biotech ADAM19 ELISA Kit (Human) (GWB-KBBDX1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAM19 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAM19 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAM19 captured in the well.
Kit Component
ComponentAmount
Anti-ADAM19 Microplate96 Wells (12 x 8 Well Strips)
ADAM19 Lyophilized Standard2 x 20 ng
100X Biotinylated ADAM19 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_150377
Nucleotide Accession Num NM_033274
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBDX1
Species Reactivity Human