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96 Wells

Additional Information

Kit Range 0.156-10 ng/mL
Sensitivity <0.064ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol ADAMTS5
Gene id 304135
Swissprot ID Q6TY19
Protein Name Submitted name: ADAMTS5
Kit Principle Genway Biotech ADAMTS5 ELISA Kit (Rat) (GWB-KBBTF2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAMTS5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAMTS5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAMTS5 captured in the well.
Kit Component
Anti-ADAMTS5 Microplate96 Wells (12 x 8 Well Strips)
ADAMTS5 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ADAMTS5 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_942056.1
Nucleotide Accession Num NM_198761.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBTF2
Species Reactivity Rat