ADCY2 ELISA Kit (Human) (GWB-KBBUX5)

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GWB-KBBUX5

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Additional Information

Name ADCY2 ELISA Kit (Human) (GWB-KBBUX5)
Description of Target Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling.
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.126 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ADCY2
Gene id 108
Alias Symbols AC2, Adenylate cyclase type 2, Adenylate cyclase type II, Adenylyl cyclase 2, ATP pyrophosphate-lyase 2, FLJ16822, FLJ45092, HBAC2, KIAA1060, MGC133314
Swissprot ID Q08462
Protein Name Adenylate cyclase type 2
Kit Principle Genway Biotech ADCY2 ELISA Kit (Human) (GWB-KBBUX5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADCY2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADCY2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADCY2 captured in the well.
Kit Component
ComponentAmount
Anti-ADCY2 Microplate96 Wells (12 x 8 Well Strips)
ADCY2 Lyophilized Standard2 x 20 ng
100X Biotinylated ADCY2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_065433
Nucleotide Accession Num NM_020546
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBUX5
Species Reactivity Human