Adipor2 ELISA Kit (Rat) (GWB-KBBDX5) (Aviva Cat. No. OKCD00786)

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Please see item number OKCD00786 to view product details and for ordering information.


GWB-KBBDX5

Aviva Cat. No.: OKCD00786

Additional Information

Name Adipor2 ELISA Kit (Rat) (GWB-KBBDX5)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.122 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ADIPOR2
Gene id 312670
Alias Symbols PAQR2, ACDCR2, Progestin and AdipoQ Receptor Family Member II
Swissprot ID Q3YAF7
Protein Name Adiponectin receptor 2
Kit Principle Genway Biotech Adipor2 ELISA Kit (Rat) (GWB-KBBDX5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adipor2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Adipor2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adipor2 captured in the well.
Kit Component
ComponentAmount
Anti-cPgC Microplate96 Wells (12 x 8 Well Strips)
cPgC Lyophilized Standard2 x 20 ng
100X Biotinylated cPgC Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001033068
Nucleotide Accession Num NM_001037979
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBDX5
Species Reactivity Rat