ADPRH ELISA Kit (Human) (GWB-KBBYP7) (Aviva Cat. No. OKCD02011)

This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKCD02011 to view product details and for ordering information.


GWB-KBBYP7

Aviva Cat. No.: OKCD02011

Additional Information

Name ADPRH ELISA Kit (Human) (GWB-KBBYP7)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.059 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ADPRH
Gene id 141
Alias Symbols ADP-ribose-L-arginine cleaving enzyme, ARH1
Swissprot ID P54922
Protein Name [Protein ADP-ribosylarginine] hydrolase
Description of Target Catalyzes the reverse reaction of mono-ADP-ribosylation.
Kit Principle Genway Biotech ADPRH ELISA Kit (Human) (GWB-KBBYP7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADPRH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADPRH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADPRH captured in the well.
Kit Component
ComponentAmount
Anti-ADPRH Microplate96 Wells (12 x 8 Well Strips)
ADPRH Lyophilized Standard2 x 10 ng
100X Biotinylated ADPRH Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001116
Nucleotide Accession Num NM_001125
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBYP7
Species Reactivity Human