ADRB3 ELISA Kit (Human) (GWB-KBBMJ5)

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GWB-KBBMJ5

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Additional Information

Name ADRB3 ELISA Kit (Human) (GWB-KBBMJ5)
Description of Target Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.117 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ADRB3
Gene id 155
Alias Symbols ADRB3R, B3AR, Beta-3 adrenergic receptor, Beta-3 adrenoceptor, Beta-3 adrenoreceptor, BETA3AR, FLJ99960
Swissprot ID P13945
Protein Name Beta-3 adrenergic receptor
Kit Principle Genway Biotech ADRB3 ELISA Kit (Human) (GWB-KBBMJ5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADRB3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADRB3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADRB3 captured in the well.
Kit Component
ComponentAmount
Anti-ADRB3 Microplate96 Wells (12 x 8 Well Strips)
ADRB3 Lyophilized Standard2 x 20 ng
100X Biotinylated ADRB3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000016
Nucleotide Accession Num NM_000025
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBMJ5
Species Reactivity Human