ADRb3 ELISA Kit (Rat) (GWB-KBBRW9)

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GWB-KBBRW9

96 Wells
$625.00
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Additional Information

Name ADRb3 ELISA Kit (Rat) (GWB-KBBRW9)
Description of Target Binds brl373 and norepenephrine, may play a role in the adrenergic signaling pathway [rgd, feb 2006]
Kit Range 0.156-10 ng/mL
Sensitivity <0.062ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol ADRb3
Gene id 25645
Alias Symbols Adrb3r, Beta-3 adrenergic receptor, Beta-3 adrenoceptor, Beta-3 adrenoreceptor
Swissprot ID P26255
Protein Name beta-3 adrenergic receptor
Kit Principle Genway Biotech ADRb3 ELISA Kit (Rat) (GWB-KBBRW9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADRb3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADRb3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADRb3 captured in the well.
Kit Component
ComponentAmount
Anti-ADRb3 Microplate96 Wells (12 x 8 Well Strips)
ADRb3 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ADRb3 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_037240.2
Nucleotide Accession Num NM_013108.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBRW9
Species Reactivity Rat