ADRBK1 ELISA Kit (Human) (GWB-KBBEO4)

GWB-KBBEO4

96 Wells
$580.00
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Additional Information

Name ADRBK1 ELISA Kit (Human) (GWB-KBBEO4)
Availability 0
Description of Target Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.125 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ADRBK1
Gene id 156
Alias Symbols BARK, BARK1, Beta-adrenergic receptor kinase 1, BETA-ARK1, Beta-ARK-1, FLJ16718, G-protein coupled receptor kinase 2, GRK2
Swissprot ID P25098
Protein Name Beta-adrenergic receptor kinase 1
Kit Principle Genway Biotech ADRBK1 ELISA Kit (Human) (GWB-KBBEO4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADRBK1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADRBK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADRBK1 captured in the well.
Kit Component
ComponentAmount
Anti-ADRBK1 Microplate96 Wells (12 x 8 Well Strips)
ADRBK1 Lyophilized Standard2 x 20 ng
100X Biotinylated ADRBK1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001610
Nucleotide Accession Num NM_001619
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)98-105%88-104%86-101%86-101%
EDTA Plasma (n=5)78-102%91-104%90-97%95-105%
Heparin Plasma (n=5)79-93%85-102%99-105%84-92%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)84-105101
EDTA Plasma (n=5)79-9185
Heparin Plasma (n=5)97-105101
Datasheets/Manuals Printable datasheet for GWB-KBBEO4
Species Reactivity Human