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96 Wells
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Additional Information

Name AFF1 ELISA Kit (Human) (GWB-KBBIW4)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.059 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol AFF1
Gene id 4299
Alias Symbols AF4, AF-4, AF4/FMR2 family member 1, ALL1-fused gene from chromosome 4 protein, FEL, MGC134969, MLLT2, PBM1, Protein AF-4, Protein FEL, Proto-oncogene AF4
Swissprot ID P51825
Protein Name AF4/FMR2 family member 1
Description of Target This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It
Kit Principle Genway Biotech AFF1 ELISA Kit (Human) (GWB-KBBIW4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AFF1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AFF1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AFF1 captured in the well.
Kit Component
Anti-CCDC80 Microplate96 Wells (12 x 8 Well Strips)
CCDC80 Lyophilized Standard2 x 10 ng
100X Biotinylated CCDC80 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001160165
Nucleotide Accession Num NM_001166693
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBIW4
Species Reactivity Human