Afp ELISA Kit (Bovine) (GWB-KBBTT5)

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Additional Information

Name Afp ELISA Kit (Bovine) (GWB-KBBTT5)
Description of Target Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin.
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.118 ng/mL
Sample Type serum, plasma and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol AFP
Gene id 506011
Alias Symbols Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein
Swissprot ID Q3SZ57
Protein Name alpha-fetoprotein
Kit Principle Genway Biotech Afp ELISA Kit (Bovine) (GWB-KBBTT5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Afp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Afp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Afp captured in the well.
Kit Component
Anti-Pf4 Microplate96 Wells (12 x 8 Well Strips)
Pf4 Lyophilized Standard2 x 20 ng
100X Biotinylated Pf4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001029434
Nucleotide Accession Num NM_001034262
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)80-96%91-99%89-97%98-105%
EDTA Plasma (n=5)93-103%78-96%79-92%86-97%
Heparin Plasma (n=5)84-101%80-101%88-102%87-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)82-9787
EDTA Plasma (n=5)89-9693
Heparin Plasma (n=5)78-10391
Datasheets/Manuals Printable datasheet for GWB-KBBTT5
Species Reactivity Bovine