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96 Wells
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Additional Information

Name AGK ELISA Kit (Human) (GWB-KBBMM1)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.081 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol AGK
Gene id 55750
Alias Symbols Acylglycerol kinase, mitochondrial, FLJ10842, hAGK, HsMuLK, MuLK, MULK, Multiple substrate lipid kinase, Multi-substrate lipid kinase
Swissprot ID Q53H12
Protein Name Acylglycerol kinase, mitochondrial
Description of Target The protein encoded by this gene is a mitochondrial membrane protein involved in lipid and glycerolipid metabolism. The encoded protein is a lipid kinase that catalyzes the formation of phosphatidic and lysophosphatidic acids. Defects in this gene have be
Kit Principle Genway Biotech AGK ELISA Kit (Human) (GWB-KBBMM1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AGK has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AGK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AGK captured in the well.
Kit Component
Anti-AGK Microplate96 Wells (12 x 8 Well Strips)
AGK Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X AGK Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_060708.1
Nucleotide Accession Num NM_018238.3
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBMM1
Species Reactivity Human