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96 Wells
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Additional Information

Name AGK ELISA Kit (Human) (GWB-KBBNV1)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.081 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol AGK
Gene id 55750
Alias Symbols Acylglycerol kinase, mitochondrial, FLJ10842, hAGK, HsMuLK, MuLK, MULK, Multiple substrate lipid kinase, Multi-substrate lipid kinase
Swissprot ID Q53H12
Protein Name acylglycerol kinase, mitochondrial
Description of Target Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, res
Kit Principle Genway Biotech AGK ELISA Kit (Human) (GWB-KBBNV1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AGK has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AGK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AGK captured in the well.
Kit Component
Anti-PSME3 Microplate96 Wells (12 x 8 Well Strips)
PSME3 Lyophilized Standard2 x 10 ng
100X Biotinylated PSME3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_060708
Nucleotide Accession Num NM_018238
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBNV1
Species Reactivity Human