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96 Wells
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Additional Information

Name AHRR ELISA Kit (Human) (GWB-KBBRC8)
Description of Target The protein encoded by this gene participates in the aryl hydrocarbon receptor (AhR) signaling cascade, which mediates dioxin toxicity, and is involved in regulation of cell growth and differentiation. It functions as a feedback modulator by repressing Ah
Kit Range 0.156-10 ng/mL
Sensitivity < 0.062 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol AHRR
Gene id 57491
Alias Symbols AHH, AHHR, AhRR, AhR repressor, Aryl hydrocarbon receptor repressor, bHLHe77, BHLHE77, Class E basic helix-loop-helix protein 77, KIAA1234, MGC167813, MGC176630
Swissprot ID A9YTQ3
Protein Name aryl hydrocarbon receptor repressor
Kit Principle Genway Biotech AHRR ELISA Kit (Human) (GWB-KBBRC8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AHRR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AHRR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AHRR captured in the well.
Kit Component
Anti-AHRR Microplate96 Wells (12 x 8 Well Strips)
AHRR Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X AHRR Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001229341.1
Nucleotide Accession Num NM_001242412.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBRC8
Species Reactivity Human