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Additional Information

Name AHRR ELISA Kit (Human) (GWB-KBBZV3)
Description of Target Mediates dioxin toxicity and is involved in regulation of cell growth and differentiation. Represses the transcription activity of AHR by competing with this transcription factor for heterodimer formation with the ARNT and subsequently binding to the xeno
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.059 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol AHRR
Gene id 57491
Alias Symbols AHH, AHHR, AhRR, AhR repressor, Aryl hydrocarbon receptor repressor, bHLHe77, BHLHE77, Class E basic helix-loop-helix protein 77, KIAA1234, MGC167813, MGC176630
Swissprot ID A9YTQ3
Protein Name aryl hydrocarbon receptor repressor
Kit Principle Genway Biotech AHRR ELISA Kit (Human) (GWB-KBBZV3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AHRR has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AHRR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AHRR captured in the well.
Kit Component
Anti-ADPRH Microplate96 Wells (12 x 8 Well Strips)
ADPRH Lyophilized Standard2 x 10 ng
100X Biotinylated ADPRH Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001229341
Nucleotide Accession Num NM_001242412
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBZV3
Species Reactivity Human