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Additional Information

Description of Target This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.068 ng/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ALCAM
Gene id 214
Alias Symbols Activated leukocyte cell adhesion molecule, CD166, CD166 antigen, FLJ38514, MEMD, MGC71733
Swissprot ID Q13740
Protein Name CD166 antigen
Kit Principle Genway Biotech ALCAM ELISA Kit (Human) (GWB-KBBJH8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALCAM has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ALCAM is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALCAM captured in the well.
Kit Component
Anti-BDNF Microplate96 Wells (12 x 8 Well Strips)
BDNF Lyophilized Standard2 x 10 ng
100X Biotinylated BDNF Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001230209
Nucleotide Accession Num NM_001243280
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)91-98%82-96%78-104%83-92%
EDTA Plasma (n=5)93-105%89-101%88-97%80-93%
Heparin Plasma (n=5)90-103%96-105%97-105%96-105%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)92-10195
EDTA Plasma (n=5)89-10498
Heparin Plasma (n=5)86-10495
Datasheets/Manuals Printable datasheet for GWB-KBBJH8
Species Reactivity Human