ALDH6A1 ELISA Kit (Human) (GWB-KBBCV1) (Aviva Cat. No. OKCD01845)

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GWB-KBBCV1

Aviva Cat. No.: OKCD01845

Additional Information

Name ALDH6A1 ELISA Kit (Human) (GWB-KBBCV1)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.058 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ALDH6A1
Gene id 4329
Alias Symbols Aldehyde dehydrogenase family 6 member A1, MGC40271, MMSADHA, MMSDH
Swissprot ID Q02252
Protein Name Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
Description of Target Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA.
Kit Principle Genway Biotech ALDH6A1 ELISA Kit (Human) (GWB-KBBCV1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALDH6A1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ALDH6A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALDH6A1 captured in the well.
Kit Component
ComponentAmount
Anti-ALDH6A1 Microplate96 Wells (12 x 8 Well Strips)
ALDH6A1 Lyophilized Standard2 x 10 ng
100X Biotinylated ALDH6A1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001265522
Nucleotide Accession Num NM_001278593
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)87-101%78-105%88-96%79-101%
EDTA Plasma (n=5)94-103%84-104%93-102%79-101%
Heparin Plasma (n=5)83-101%88-102%94-105%79-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)83-9693
EDTA Plasma (n=5)91-9995
Heparin Plasma (n=5)82-10194
Datasheets/Manuals Printable datasheet for GWB-KBBCV1
Species Reactivity Human