Alk-Smase ELISA Kit (Mouse) (GWB-KBBLV1)


96 Wells
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Additional Information

Name Alk-Smase ELISA Kit (Mouse) (GWB-KBBLV1)
Availability In Stock
Kit Range 0.156-10 ng/mL
Sensitivity <0.051 ng/mL
Sample Type Serum, plasma, tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol Alk-Smase
Gene id 238011
Alias Symbols Alk-SMase, Gm254, LOC238011
Swissprot ID Q3TIW9
Protein Name ectonucleotide pyrophosphatase/phosphodiesterase family member 7
Kit Principle Genway Biotech Alk-Smase ELISA Kit (Mouse) (GWB-KBBLV1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Alk-Smase has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Alk-Smase is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alk-Smase captured in the well.
Kit Component
Anti-Alk-Smase Microplate96 Wells (12 x 8 Well Strips)
Alk-Smase Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X Alk-Smase Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num XP_006533321.1
Nucleotide Accession Num XM_006533258.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBLV1
Species Reactivity Mouse