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Alk-Smase ELISA Kit (Rat) (GWB-KBBCL3)

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96 Wells
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Additional Information

Name Alk-Smase ELISA Kit (Rat) (GWB-KBBCL3)
Description of Target Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity.
Kit Range 0.156-10 ng/mL
Sensitivity < 0.043 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol Alk-Smase
Gene id 303729
Alias Symbols Alkaline sphingomyelin phosphodiesterase, Alk-SMase, Ectonucleotide pyrophosphatase/phosphodiesterase family member 7, E-NPP 7, Intestinal alkaline sphingomyelinase, NPP-7
Swissprot ID Q5EZ72
Protein Name ectonucleotide pyrophosphatase/phosphodiesterase family member 7
Kit Principle Genway Biotech Alk-Smase ELISA Kit (Rat) (GWB-KBBCL3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Alk-Smase has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Alk-Smase is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alk-Smase captured in the well.
Kit Component
Anti-Alk-Smase Microplate96 Wells (12 x 8 Well Strips)
Alk-Smase Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X Alk-Smase Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001012484.1
Nucleotide Accession Num NM_001012466.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBCL3
Species Reactivity Rat