Ambp ELISA Kit (Mouse) (GWB-KBBAW2) (Aviva Cat. No. OKCD00654)

This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKCD00654 to view product details and for ordering information.


GWB-KBBAW2

Aviva Cat. No.: OKCD00654

Additional Information

Name Ambp ELISA Kit (Mouse) (GWB-KBBAW2)
Kit Range 1.56 - 100 ng/mL
Sensitivity < 0.67 ng/mL
Sample Type serum, plasma and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol AMBP
Gene id 11699
Alias Symbols AI194774, ASPI, HI-30, Intin4, Itil, Protein AMBP, ulinastatin, Urinary Trypsin Inhibitor, UTI
Swissprot ID Q07456
Protein Name protein AMBP
Description of Target Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization (
Kit Principle Genway Biotech Ambp ELISA Kit (Mouse) (GWB-KBBAW2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ambp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ambp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ambp captured in the well.
Kit Component
ComponentAmount
Anti-Tfr2 Microplate96 Wells (12 x 8 Well Strips)
Tfr2 Lyophilized Standard2 x 100 ng
100X Biotinylated Tfr2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_031469
Nucleotide Accession Num NM_007443
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)87-97%80-90%78-103%78-95%
EDTA Plasma (n=5)83-101%78-98%78-101%87-95%
Heparin Plasma (n=5)80-91%84-101%84-92%96-105%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)83-10398
EDTA Plasma (n=5)99-105102
Heparin Plasma (n=5)79-9185
Datasheets/Manuals Printable datasheet for GWB-KBBAW2
Species Reactivity Mouse