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Androstenedione ELISA Kit (GWB-48E513)



1x96 well plate
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Product Name: Androstenedione

Category: Fertility

Sample Type: Serum and plasma

Sensitivity: 0,043 ng/mL

Description: A direct competitive solid phase enzyme immunoassay, with a peroxidase conjugate as label and tetramethylbenzidine (TMB) as chromogen.

Introduction: The Androstenedione Enzyme Immunoassay Kit provides materials for the quantitative determination of Androstenedione in serum and EDTA plasma.
This assay is intended for research use only.
The steroid hormone Androstenedione is one of the main androgens, besides Testosterone and Dehydroepiandrosterone. Testosterone, the most important biological active androgen, is derived from peripheral enzymatic conversion of Androstenedione.
In males, androgens are secreted primarily by the Leydig cells of the testes, to some degree also in the adrenal cortex. In females, the androgens are secreted mainly in the adrenal glands and in the ovary. Around 10% of the androgens are derived from peripheral conversion, mainly of DHEA. Androstenedione and Testosterone show high diurnal variability. The highest levels are measured in the morning. At the age of puberty serum androstenedione levels rise, after menopause they decline again. High androstenedione levels are measured during pregnancy. In women, high levels of androstenedione (47-100% above normal) are generally found in hirsutism, mostly in combination with other androgens as testosterone and DHEA-S. Androstenedione overproduction is due to ovarian dysfunction or maybe of adrenal origin. High circulating androstenedione levels are found in women with polycystic ovaries and 21-hydroxylase effect. Significant lower androstenedione levels are found in postmenopausal osteoporosis.

Principle of the test: The GenWay Androstenedione ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding.
The microtiter wells are coated with an antibody directed towards an antigenic site on the Androstenedione molecule. Endogenous Androstenedione of a patient sample competes with an Androstenedione horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off.
The amount of bound peroxidase conjugate is reverse proportional to the concentration of Androstenedione in the sample. After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of Androstenedione in the patient sample.

Storage and Stability: When stored at 2-8C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
Opened reagents must be stored at 2-8C. Microtiter wells must be stored at 2-8C. Once the foil bag has been opened, care should be taken to close it tightly again. Opened kits retain activity for three months if stored as described above.

Product Name: Androstenedione

Additional Information

Name Androstenedione ELISA Kit (GWB-48E513)
Related Product Names Androstenedione ELISA Kit Androstenedione
Datasheets/Manuals Download manual for GWB-48E513
Intended Use Research Use Only