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96 Wells
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Additional Information

Description of Target Binds to tek/tie2, competing for the angpt1 binding site, and modulating angpt1 signaling. can induce tyrosine phosphorylation of tek/tie2 in the absence of angpt1. in the absence of angiogenic inducers, such as vegf, angpt2-mediated loosening of cell-mat
Kit Range 0.156-10 ng/mL
Sensitivity <0.054 ng/mL
Sample Type Serum, plasma
Kit Detection Absorbance, OD 450 nm
Gene Symbol ANGPT2
Gene id 89805
Alias Symbols Agpt2, Ang-2, ANG-2, Angiopoietin-2
Swissprot ID O35462
Protein Name angiopoietin-2
Kit Principle Genway Biotech ANGPT2 ELISA Kit (Rat) (GWB-KBBOW6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANGPT2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANGPT2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ANGPT2 captured in the well.
Kit Component
Anti-ANGPT2 Microplate96 Wells (12 x 8 Well Strips)
ANGPT2 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ANGPT2 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_604449.1
Nucleotide Accession Num NM_134454.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBOW6
Species Reactivity Rat