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Additional Information

Name ANLN ELISA Kit (Human) (GWB-KBBJE1)
Description of Target Required for cytokinesis.
Kit Range 31.2 - 2,000 pg/mL
Sensitivity < 11.5 pg/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol ANLN
Gene id 54443
Alias Symbols Actin-binding protein anillin, ANILLIN, DKFZp779A055, scra, Scraps
Swissprot ID Q9NQW6
Protein Name Actin-binding protein anillin
Kit Principle Genway Biotech ANLN ELISA Kit (Human) (GWB-KBBJE1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANLN has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANLN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ANLN captured in the well.
Kit Component
Anti-ANLN Microplate96 Wells (12 x 8 Well Strips)
ANLN Lyophilized Standard2 x 2 ng
100X Biotinylated ANLN Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001271230
Nucleotide Accession Num NM_001284301
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)79-95%90-102%91-103%88-96%
EDTA Plasma (n=5)97-104%91-105%84-98%79-96%
Heparin Plasma (n=5)95-105%78-105%78-94%80-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)82-9486
EDTA Plasma (n=5)96-105101
Heparin Plasma (n=5)78-9790
Datasheets/Manuals Printable datasheet for GWB-KBBJE1
Species Reactivity Human