Description
Short Description: MOUSE ANTI RAT MHC CLASS I RT1A
Additional Information
| Name | Rat MHC Class I Antibody (Rt1A) (GWB-1002F1) |
|---|---|
| Related Product Names | MOUSE ANTI RAT MHC CLASS I RT1A MHC CLASS I RT1A |
| Description of Target | MOUSE ANTI RAT MHC CLASS I RT1A |
| Immunogen | Rat spleen glycoproteins |
| Gene Symbol | RT1-A1 |
| Gene Fullname | RT1 class I, A1 |
| Gene id | 24973 |
| Swissprot ID | Q6MGB9 |
| Protein Name | RT1 class I, A1 |
| Protein Accession Num | NP_001008827.1 |
| Nucleotide Accession Num | NM_001008827.1 |
| Clone | OX-18 |
| Host | Mouse |
| Isotype | IgG1 |
| Homology | Rat |
| NCBI Acc Number | NP_620235.1 |
| Product Format | Purified IgG - liquid |
| Specificity | MHC CLASS I RT1A |
| Concentration | 1mg/ml |
| Application | C*, E, F, IP |
| Application Data | Application #1: Staining of rat spleen cells with Mouse anti Rat MHC Class I (RT1A) Monomorphic: FITC  |
| GI Number | 20302055 |
| Clonality | Monoclonal |
| Format | Solution: Phosphate buffered saline, Stabalizer: 0.09%, Sodium Azide, Form: Purified IgG - liquid |
| Stability | 18 months from date of despatch. |
| Reconstitution and Storage | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Reactivity | Rat |
| Application Info | Flow Cytometry: 1/50 - 1/100 |
| Storage | Store at +4C or at -20C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate |
| Datasheets/Manuals | Printable datasheet for GWB-1002F1 |
| Species Reactivity | Rat |
| Intended Use | Research Use Only |
| Key Reference | 1. Fukumoto, T. et al. (1982) Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and Class I antigens in rat thymus. Eur. J. Immunol. 12: 237-243. 2. Bukovsk½, A. et al. (1984) Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy. Immunology. 52:631-640 3. Osawa, H. et al. (1985) Inhibition of IL 2-dependent proliferation of rat T lymphoblasts by the monoclonal antibody ART62 which reacts with MHC class 1 antigens J. Immunol. 134(6): 3901-3906. 4. Stet, R.J.M. et al. (1987) U9F4: A monoclonal antibody recognizing a rat polymorphic class I determinant. Transpl. Proc. 19: 3004-3005. 5. Fujikawa, L. S. et al. (1989) Class II antigens on retinal vascular endothelium, pericytes, macrophages, and lymphocytes of the rat Invest. Ophthalmol. Vis. Sci. 30(1): 66-73 6. Zhai, Y. and Knechtle, S. et al. (1998) Two distinct forms of soluble MHC class I molecules synthesized by different mechanisms in normal rat cells in vitro Human Immunol. 59: 404-414 |
| Additional Information | Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line. |
| :: | Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant Preservative Stabilisers: 0.09% - Sodium Azide |
| :: | Approx Protein Conc: IgG concentration 1.0 mg/ml Buffer Solutions: Phosphate buffered saline pH7.4 |