Mouse MHC Class I H-2Kd, Antibody

Isotype: IgG3.k

Clone: A5A1-Do4

Host Animal: Mouse

Specificity: MHC Class I H-2Kd.Dd.q.u.v Type of Product: Monoclonal Antibodies to Murine-Rat Major Histocompatibility Antigens

Concentration: 0.5mg/ml (OD280)

Preservatives: NaN3

Buffer: 0.01M PBS, pH 7.2 containing 1% BSA

Applications Notes : Cell surface analysis, genetic mapping, allele specific cell elimination, FACS analysis, complement-dependent microcytoxicity killing, immunoprecipitation. Functional studies: blocking CTLs (class I-specific). We recommend using 1ug to stain 1 x 106 cells in flow cytometric applications. Depending on the particular application, some variance can be expected. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Warning: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1–1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. MAb to Mouse MHC Class I H-2Kd. Monoclonal Antibody to Mouse MHC Class I H-2Kd, Dd,q,u

Function: Involved in the presentation of foreign antigens to the immune system.

Subunit: Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin).

Subcellular Location: Membrane; Single-pass type I membrane protein.

Similarity: Belongs to the MHC class I family.

Similarity: Contains 1 Ig-like C1-type (immunoglobulin-like) domain.