Name |
ospC Antibody (GWB-54XYPC) |
Gene Symbol |
ospC |
Gene Fullname |
Outer surface protein C |
Swissprot ID |
Q08137 |
Protein Name |
Outer surface protein C |
Clone |
Polyclonal |
Host |
Rabbit |
Homology |
Borrelia burgdorferi |
Product Format |
Liquid (sterile filtered) |
Purification |
This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. |
Specificity |
This antibody is specific for Borrelia burgdorferi OspC protein. A BLAST analysis was used to suggest cross-reactivity with p39 from B. burgdorferi, afzelii, and valaisiana sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. japonica and americana sources based on 94% homology. Cross-reactivity with OspC from other sources has not been determined. |
Concentration |
1.0 mg/mL (by UV absorbance at 280 nm) |
Application |
ELISA, WB |
Application Data |
This protein-A purified antibody has been tested for use in Western blotting and ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 20.7 kDa in size corresponding to Borrelia burgdorferi OspC protein by Western blotting in the appropriate cell lysate or extract. |
Clonality |
Polyclonal |
Reconstitution and Storage |
Store vial at -20C or below prior to opening. This vial contains a relatively low volume of reagent (25 uL). To minimize loss of volume dilute 1:10 by adding 225 uL of the buffer stated below directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20C or below after dilution. Avoid cycles of freezing and thawing. Expiration date is three (3) months from date of opening. |
Datasheets/Manuals |
Printable datasheet for GWB-54XYPC |
Species Reactivity |
Borrelia burgdorferi |