Rat Anti-Mouse CD24/HSA Antibody (GWB-F7AF55) (Aviva Cat. No. OASB00630)

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GWB-F7AF55

Aviva Cat. No.: OASB00630

20-787-275652

Description

CD24 is expressed in B cells until their terminal differentiation into plasma cells. It has been used widely as a marker to fractionate different B-lineage differentiation stages. CD24 has been found on a variety of other hematopoietic and neural cell types and is now classified as a costimulatory molecule. The CD24 molecule, also known as HSA, is composed mostly of carbohydrate with a protein core of only 31-35 amino acids in the human and 27 amino acids in the mouse. CD24 is now included in the large group of phosphatidylinositol-anchored membrane proteins. Studies with purified CD24 applied to latex beads have demonstrated a self-binding property suggesting that the molecule is capable of promoting homotypic aggregation, binding, and cell adhesion. This is supported by earlier observations of the blockade of B cell lymphoblast aggregation with an anti-CD24 monoclonal antibody. Recent studies of murine CD24 have assigned a role for CD24 as a ligand for P-selectin, a calcium-dependent lectin.

Additional Information

Name Rat Anti-Mouse CD24/HSA Antibody (GWB-F7AF55)
Related Product Names Rat Anti-Mouse CD24/HAS-APC; Heat-stable antigen; HSA; Nectadrin Cd24aRat Anti-Mouse CD24/HSACd24
Gene Symbol CD24
Gene Fullname Signal transducer CD24
Gene id 100133941
Swissprot ID P25063
Protein Name Signal transducer CD24
Protein Accession Num NP_001278666.1; NP_001278667.1; NP_037362.1
Nucleotide Accession Num NM_001291737.1; NM_001291738.1; NM_013230.3
Clone 30- F1
Application Info FC: ≤ 0.2 ug/106 cells. For FC, the suggested use of these reagents is in a final volume of 100 uL.
Host Rat
Immunogen Mouse thymus or spleen
Isotype Rat (LOU/Ws1/M) IgG2cK
Conjugation Allophycocyanin (APC) Conjugate
NCBI Acc Number CAA77731.1
Product Format Liquid PBS containing < 0.1% sodium azide and a stabilizer
Specificity Mouse CD24/heat stable antigen (HSA)
Concentration 0.1 mg/ml
Application FC
GI Number 55902
Clonality Monoclonal
Reconstitution and Storage Store at 2-8°C. Avoid exposure to light; do not freeze.
Reactivity Mouse
Sequence Length 76
Warning Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact withskin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, washwith copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azideyields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running waterbefore discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Datasheets/Manuals Printable datasheet for GWB-F7AF55
Protocol
Characterization To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
Lead Time Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Intended Use Research Use Only
Key Reference 1. Kay, R., P. M. Rosten and R. K. Humphries. 1991. J. Immunol. 147:1412- 1416
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3. Hubbe, M. and P. Altevogt. 1994. Eur. J. Immunol. 1994. 24:731
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5. Shields, J. G., S. H. Smith and R. E. Callard. 1987. In Leukocyte Typing III. A. J. McMichael, et. al., eds., Oxford University Press, Oxford. P. 488
6. Kadmon, G., M. Eckert, M. Sammar, M. Schachner, and P. Altevogt. 1992. J. Cell Biol. 118:1245
7. Aigner, S., M. Ruppert, M. Hubbe, M. Sammar, Z. Sthoeger, E. C. Butcher, D. Vestweber, and P. Altevogt. 1995. International Immunology 7:1557
8. Ledbetter, J. A., and L. A. Herzenberg. 1979. Immunol. Rev. 47: 63
9. Kariv, I., R. R. Hardy and K. Hayakawa. 1994. J. Exp. Med. 180:2419
10. Hardy, R. R., C. E. Carmack, S. A. Shinton, J. D. Kemp, and K. Hayakawa. 1991. J. Exp. Med 173:1213.