Mouse CD68 Antibody (GWB-Q01478) (Aviva Cat. No. OASA05369)

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GWB-Q01478

Aviva Cat. No.: OASA05369

Additional Information

Name Mouse CD68 Antibody (GWB-Q01478)
Related Product Names RAT ANTI MOUSE CD68 Antibody, Monoclonal Antibody, RAT ANTI MOUSE CD68
Gene Symbol Cd68
Gene Family CD
Gene id 12514
Alias Symbols gp110, Scard1, Cd68
Swissprot ID P31996
Protein Name Macrosialin
Description of Target CD68 is considered a pan macrophage marker, predominantly expressed on the intracellular lysosomes of tissue macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes. CD68 is expressed by many tumor types including some B cell lymphomas, blastic NK lymphomas, melanomas, granulocytic (myeloid) sarcomas, hairy cell leukemias, and renal, urinary and pancreatic tumors, and can be used in cancer studies to demonstrate the presence/localization of macrophages.
Rat anti mouse CD68 antibody, clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical studies, using both frozen and paraffin-embedded tissues (Masaki et al. 2003) and (Devey et al. 2009).
Rat anti mouse CD68 antibody, clone FA-11 can be used in flow cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Protein Accession Num NP_033983.1
Clone FA-11
Application Info FC: 1/50 - 1/100. Use 10 uL of the suggested working dilution to label 106 cells in 100 uL. Membrane permeabilisation is required for this product.
IHC-P: This product may require antigen retrieval using heat treatment prior to staining of paraffin sections. Either sodium citrate buffer or Tris/EDTA buffer may be used for this purpose.
WB: Non-reducing conditions recommended.
Host Rat
Immunogen Purified Concanavalin A acceptor glycoprotein from P815 cell line
Isotype IgG2a
Replacement This antibody may replace item sc-17832 from Santa Cruz Biotechnology.
Homology Mouse
Product Format Liquid PBS with 0.09% sodium azide
Purification Affinity chromatography on Protein G from tissue culture supernatant
Specificity Rat anti Mouse CD68 antibody, clone FA-11 recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, which is classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain.
Concentration 1.0 mg/ml
Application FC, IHC-F, IHC-P, IP, WB, IF
Stability 12 months from date of dispatch.
Reconstitution and Storage Store at 4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Reactivity Mouse
Datasheets/Manuals Printable datasheet for GWB-Q01478
Protocol Information Citation: 1: Sun Y, Ran H, Zamzow M, Kitatani K, Skelton MR, Williams MT, Vorhees CV, Witte DP, Hannun YA, Grabowski GA. Specific saposin C deficiency: CNS impairment and acid beta-glucosidase effects in the mouse. Hum Mol Genet. 2010 Feb 15;19(4):634-47. Epub 2009 Dec 16. PubMed PMID: 20015957; PubMed Central PMCID: PMC2807372.
Species: Saposin C-deficient mice
Experiment Name: Histological studies
Experiment Background: The structure of saposin C is restrained by three disulfide bonds. Biophysical studies have shown that saposin C has membrane fusion properties. This fusogenic property is thought to be involved in the maturation of multivesicular bodies. Dissection of the saposin C peptide and in vitro and in vivo studies indicate regional localization of these multiple functions to specific regions of the protein, including neuritogenic activity and GCase activation. The fusogenic and neuritogenic activities localize to the 40 amino acids of the N-terminal, whereas the COOH-half contains the GCase activation domain. The former functions can be duplicated with the appropriate saposin C peptide fragments. In comparison, activation of GCase requires the COOH-half within a ‘saposin’ structure with intact disulfide bonds. The N-terminal 35–40 amino acid sequence is not critical to this GCase activation function, as the corresponding peptide sequence from saposin B can fulfill the overall requirements.
Experimental Steps: Following CO2 narcosis, mice were perfused with saline and then 4% paraformaldehyde.2.The tissues were dissected and fixed in 10% formalin, embedded in paraffin, sectioned and stained with hemotoxilin and eosin (H&E). 1. Paraffin sections of cerebellum were stained with mouse anti-calbindin D28K and mouse anti-GFAP. The antibodies were diluted in PBS. 2. Detection was done with ABC Vectastain and the alkaline phosphatase kit II (Black). 3. The slides were counterstained with methyl green. 4. For immunofluorescence staining, frozen sections from paraformaldhyde fixed tissues were incubated with mouse anti-GFAP. 5. Paraffin sections were reacted with mouse anti-NeuN. 6. The antibodies were diluted in PBS7.. Both biotinylated goat anti-mouse and streptavidin-conjugated fluorescent 480 (green) were applied to the sections. The samples were counterstained with antifade/DAPI. 8. The signals were visualized with a Zeiss Axiovert 200M microscope equipped with an Apotome. CD68 monoclonal antibody (FA-11) staining on the frozen sections was done.9. Karnovsky's fixative was used for ultrastructural studies.
Number Of Protocols: 1
Species Reactivity Mouse
Lead Time Domestic: within 2-3 weeks delivery | International: 2-3 weeks
Intended Use Research Use Only
Key Reference 1. Ramprasad, M.P. et al. (1996) Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low density lipoprotein. Proc. Natl. Acad. Sci. 93: 14833-14838.
2. Rabinowitz, S.S. & Gordon, S. (1991) Macrosialin, a macrophage-restricted membrane sialoprotein differentially glycosylated in response to inflammatory stimuli. J. Exp. Med. 174: 827-836.
3. Da Silva, R.P & Gordon, S. (1999) Phagocytosis stimulates alternative glycosylation of macrosialin (mouse CD68), a macrophage-specific endosomal protein. Biochem. J. 338: 687-694.
4. Schleicher, U. et al. (2005) Minute numbers of contaminant CD8+ T cells or CD11b+CD11c+ NK cells are the source of IFN-{gamma} in IL-12/IL-18-stimulated mouse macrophage populations. Blood 105: 1319-1328.
5. von Lukowicz, T. et al. (2008) PARP1 is required for adhesion molecule expression in atherogenesis. Cardiovasc. Res. 78: 158-166