Fluorescein Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (RABBIT)

GWB-4A7B1C

25-732-294316

2 mg
$179.00
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Description

Antigen: This antibody is also available conjugated to biotin and FITC. MHC Class II is consists of 2 membrane spanning proteins. Each of these proteins is roughly 30 kDa, and has of two Globular domains, Alpha-1, Alpha-2, Beta-1 and Beta-2. The two regions farthest away from the membrane are alpha-1 and beta-1. The two proteins link without Covalent Bonds. The MHC Class II protein mainly presents peptides, which have been digested from external sources. MHC Class II is normally only expressed by Antigen Presenting Cells (APC) which digest foreign protein. Within the RER the alpha and beta proteins of the molecule, associate with each other, while a third protein called the "invariant chain," is necessary for stabilizing the complex, without it, they will not associate. The MHC-invariant complex is then passed from the RER, into and out of, the Golgi body. It fuses with an Endocytic compartment, where an external protein has been sampled and degraded.

Buffer: This antibody is also available conjugated to biotin and FITC. MHC Class II is consists of 2 membrane spanning proteins. Each of these proteins is roughly 30 kDa, and has of two Globular domains, Alpha-1, Alpha-2, Beta-1 and Beta-2. The two regions farthest away from the membrane are alpha-1 and beta-1. The two proteins link without Covalent Bonds. The MHC Class II protein mainly presents peptides, which have been digested from external sources. MHC Class II is normally only expressed by Antigen Presenting Cells (APC) which digest foreign protein. Within the RER the alpha and beta proteins of the molecule, associate with each other, while a third protein called the "invariant chain," is necessary for stabilizing the complex, without it, they will not associate. The MHC-invariant complex is then passed from the RER, into and out of, the Golgi body. It fuses with an Endocytic compartment, where an external protein has been sampled and degraded.

Fraction: This antibody is also available conjugated to biotin and FITC. MHC Class II is consists of 2 membrane spanning proteins. Each of these proteins is roughly 30 kDa, and has of two Globular domains, Alpha-1, Alpha-2, Beta-1 and Beta-2. The two regions farthest away from the membrane are alpha-1 and beta-1. The two proteins link without Covalent Bonds. The MHC Class II protein mainly presents peptides, which have been digested from external sources. MHC Class II is normally only expressed by Antigen Presenting Cells (APC) which digest foreign protein. Within the RER the alpha and beta proteins of the molecule, associate with each other, while a third protein called the "invariant chain," is necessary for stabilizing the complex, without it, they will not associate. The MHC-invariant complex is then passed from the RER, into and out of, the Golgi body. It fuses with an Endocytic compartment, where an external protein has been sampled and degraded.

Additional Information

Name Fluorescein Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (RABBIT)
Availability In Stock
Related Product Names Fluorescein Conjugated Affinity Purified anti-Mouse IgG [H&L] [Rabbit]; N/A Fluorescein Conjugated Affinity Purified Anti-MOUSE IgG (H&L) (RABBIT)IGHG1
Alias Symbols FITC
Swissprot ID P01857
Clone Polyclonal
Immunogen Mouse IgG whole molecule
Product Format Lyophilized
Molecular Weight 390
Application WB, ELISA, IHC, Fluorescence Microscopy, FACS
Application Info IF: 1:500 - 1:2,500 OTHER: FLOW CYTOMETRY 1:2,000 - 1:10,000
Format Lyophilized
Stability expiration date is one year from date of opening
Reconstitution and Storage Store vial at 4 C prior to restoration.     Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20 C or below.   Avoid cycles of freezing and thawing.   Centrifuge product if not completely clear after standing at room temperature.   This product is stable for several weeks at 4 C as an undiluted liquid.   Dilute only prior to immediate use.     Expiration date is one (1) year from date of restoration.
Reactivity Mouse IgG
Storage 4C prior to restoration. for extended storage aliquot and freeze at -20C or below
Datasheets/Manuals Printable datasheet for GWB-4A7B1C
Intended Use Research Use Only
Additional Information Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Color: Green
Conjugation Reference: Modified from The and Feltkamp, Immunology 18; 865, 1970.
Emission Wavelength: 528
Excitation Wavelength: 495
Fluorochrome/Protein Ratio: 3.1 moles FITC per mole of Rabbit IgG
:: Label: Fluorescein (FITC)
Preservative: 0.01% (w/v) Sodium Azide
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
:: Description: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.