AP2m1 ELISA Kit (Human) (GWB-KBBVY4)

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96 Wells
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Additional Information

Name AP2m1 ELISA Kit (Human) (GWB-KBBVY4)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.057 ng/mL
Sample Type Serum, plasma, tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol AP2m1
Gene id 1173
Alias Symbols Adapter-related protein complex 2 mu subunit, Adaptin-mu2, Adaptor protein complex AP-2 subunit mu, AP-2 complex subunit mu, AP-2 mu chain, AP50, CLAPM1, Clathrin assembly protein complex 2 medium chain, Clathrin coat assembly protein AP50, Clathrin coat-
Swissprot ID Q96CW1
Protein Name AP-2 complex subunit mu
Description of Target This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proto
Kit Principle Genway Biotech AP2m1 ELISA Kit (Human) (GWB-KBBVY4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AP2m1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AP2m1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AP2m1 captured in the well.
Kit Component
Anti-AP2m1 Microplate96 Wells (12 x 8 Well Strips)
AP2m1 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X AP2m1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001020376.1
Nucleotide Accession Num NM_001025205.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBVY4
Species Reactivity Human