AP2M1 ELISA Kit (Human) (GWB-KBBXH8)

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GWB-KBBXH8

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Additional Information

Name AP2M1 ELISA Kit (Human) (GWB-KBBXH8)
Description of Target Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in ca
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.057 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol AP2M1
Gene id 1173
Alias Symbols Adapter-related protein complex 2 mu subunit, Adaptin-mu2, Adaptor protein complex AP-2 subunit mu, AP-2 complex subunit mu, AP-2 mu chain, AP50, CLAPM1, Clathrin assembly protein complex 2 medium chain, Clathrin coat assembly protein AP50, Clathrin coat-
Swissprot ID Q96CW1
Protein Name AP-2 complex subunit mu
Kit Principle Genway Biotech AP2M1 ELISA Kit (Human) (GWB-KBBXH8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AP2M1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AP2M1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AP2M1 captured in the well.
Kit Component
ComponentAmount
Anti-APLP2 Microplate96 Wells (12 x 8 Well Strips)
APLP2 Lyophilized Standard2 x 10 ng
100X Biotinylated APLP2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001020376
Nucleotide Accession Num NM_001025205
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBXH8
Species Reactivity Human