AP3B1 ELISA Kit (Human) (GWB-KBBXD2)

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GWB-KBBXD2

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Additional Information

Name AP3B1 ELISA Kit (Human) (GWB-KBBXD2)
Description of Target Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.064 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol AP3B1
Gene id 8546
Alias Symbols Adapter-related protein complex 3 subunit beta-1, Adaptor protein complex AP-3 subunit beta-1, ADTB3, ADTB3A, AP-3 complex subunit beta-1, Beta-3A-adaptin, Clathrin assembly protein complex 3 beta-1 large chain, HPS, HPS2, PE
Swissprot ID O00203
Protein Name AP-3 complex subunit beta-1
Kit Principle Genway Biotech AP3B1 ELISA Kit (Human) (GWB-KBBXD2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AP3B1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AP3B1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AP3B1 captured in the well.
Kit Component
ComponentAmount
Anti-SUMO1 Microplate96 Wells (12 x 8 Well Strips)
SUMO1 Lyophilized Standard2 x 10 ng
100X Biotinylated SUMO1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001258698
Nucleotide Accession Num NM_001271769
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
82-93%90-102%81-101%96-103%
Kit Recovery
MatrixRecovery Range (%)Average (%)
78-10397
Datasheets/Manuals Printable datasheet for GWB-KBBXD2
Species Reactivity Human