Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and
Kit Range
0.156 - 10 ng/mL
Sensitivity
< 0.064 ng/mL
Sample Type
tissue homogenates, cell lysates and other biological fluids
Genway Biotech AP3B1 ELISA Kit (Human) (GWB-KBBXD2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AP3B1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AP3B1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AP3B1 captured in the well.
