| Name |
AP3B1 ELISA Kit (Human) (GWB-KBBXD2) |
| Description of Target |
Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and |
| Kit Range |
0.156 - 10 ng/mL |
| Sensitivity |
< 0.064 ng/mL |
| Sample Type |
tissue homogenates, cell lysates and other biological fluids |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
AP3B1 |
| Gene id |
8546 |
| Alias Symbols |
Adapter-related protein complex 3 subunit beta-1, Adaptor protein complex AP-3 subunit beta-1, ADTB3, ADTB3A, AP-3 complex subunit beta-1, Beta-3A-adaptin, Clathrin assembly protein complex 3 beta-1 large chain, HPS, HPS2, PE |
| Swissprot ID |
O00203 |
| Protein Name |
AP-3 complex subunit beta-1 |
| Kit Principle |
Genway Biotech AP3B1 ELISA Kit (Human) (GWB-KBBXD2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AP3B1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AP3B1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AP3B1 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-SUMO1 Microplate | 96 Wells (12 x 8 Well Strips) | | SUMO1 Lyophilized Standard | 2 x 10 ng | | 100X Biotinylated SUMO1 Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_001258698 |
| Nucleotide Accession Num |
NM_001271769 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Kit linearity |
| Sample | 1:2 Dilution | 1:4 Dilution | 1:8 Dilution | 1:16 Dilution |
|---|
| 82-93% | 90-102% | 81-101% | 96-103% | |
|
| Kit Recovery |
| Matrix | Recovery Range (%) | Average (%) |
|---|
| 78-103 | 97 | |
|
| Datasheets/Manuals |
Printable datasheet for GWB-KBBXD2 |
| Species Reactivity |
Human |
