| Name |
API5 ELISA Kit (Human) (GWB-KBBFS5) |
| Description of Target |
Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its de |
| Kit Range |
0.312 - 20 ng/mL |
| Sensitivity |
< 0.122 ng/mL |
| Sample Type |
tissue homogenates, cell lysates and other biological fluids. |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
API5 |
| Gene id |
8539 |
| Alias Symbols |
AAC11, AAC-11, Antiapoptosis clone 11 protein, API-5, API5L1, Apoptosis inhibitor 5, Cell migration-inducing gene 8 protein, Fibroblast growth factor 2-interacting factor, FIF, MIG8, Protein XAGL |
| Swissprot ID |
Q9BZZ5 |
| Protein Name |
apoptosis inhibitor 5 |
| Kit Principle |
Genway Biotech API5 ELISA Kit (Human) (GWB-KBBFS5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for API5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for API5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample API5 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-SLC12A3 Microplate | 96 Wells (12 x 8 Well Strips) | | SLC12A3 Lyophilized Standard | 2 x 20 ng | | 100X Biotinylated SLC12A3 Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_001136402 |
| Nucleotide Accession Num |
NM_001142930 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Kit linearity |
| Sample | 1:2 Dilution | 1:4 Dilution | 1:8 Dilution | 1:16 Dilution |
|---|
| 87-94% | 79-90% | 91-98% | 85-101% | |
|
| Kit Recovery |
| Matrix | Recovery Range (%) | Average (%) |
|---|
| 95-105 | 101 | |
|
| Datasheets/Manuals |
Printable datasheet for GWB-KBBFS5 |
| Species Reactivity |
Human |
