API5 ELISA Kit (Human) (GWB-KBBFS5)

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GWB-KBBFS5

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Additional Information

Name API5 ELISA Kit (Human) (GWB-KBBFS5)
Description of Target Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its de
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.122 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol API5
Gene id 8539
Alias Symbols AAC11, AAC-11, Antiapoptosis clone 11 protein, API-5, API5L1, Apoptosis inhibitor 5, Cell migration-inducing gene 8 protein, Fibroblast growth factor 2-interacting factor, FIF, MIG8, Protein XAGL
Swissprot ID Q9BZZ5
Protein Name apoptosis inhibitor 5
Kit Principle Genway Biotech API5 ELISA Kit (Human) (GWB-KBBFS5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for API5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for API5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample API5 captured in the well.
Kit Component
ComponentAmount
Anti-SLC12A3 Microplate96 Wells (12 x 8 Well Strips)
SLC12A3 Lyophilized Standard2 x 20 ng
100X Biotinylated SLC12A3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001136402
Nucleotide Accession Num NM_001142930
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
87-94%79-90%91-98%85-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
95-105101
Datasheets/Manuals Printable datasheet for GWB-KBBFS5
Species Reactivity Human