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Apoa4 ELISA Kit (Mouse) (GWB-KBBYU3)

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96 Wells
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Additional Information

Name Apoa4 ELISA Kit (Mouse) (GWB-KBBYU3)
Kit Range 1.56 - 100 ng/mL
Sensitivity < 0.69 ng/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol APOA4
Gene id 11808
Alias Symbols Apoa-4, ApoA-IV, Apo-AIV, Apolipoprotein A4, Apolipoprotein A-IV
Swissprot ID P06728
Protein Name apolipoprotein A-IV
Description of Target May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
Kit Principle Genway Biotech Apoa4 ELISA Kit (Mouse) (GWB-KBBYU3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apoa4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Apoa4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apoa4 captured in the well.
Kit Component
Anti-TERF1 Microplate96 Wells (12 x 8 Well Strips)
TERF1 Lyophilized Standard2 x 100 ng
100X Biotinylated TERF1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_031494
Nucleotide Accession Num NM_007468
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)86-99%82-96%92-101%78-98%
EDTA Plasma (n=5)78-102%93-102%79-101%97-105%
Heparin Plasma (n=5)87-102%93-105%91-105%90-102%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)84-10295
EDTA Plasma (n=5)80-9184
Heparin Plasma (n=5)89-10398
Datasheets/Manuals Printable datasheet for GWB-KBBYU3
Species Reactivity Mouse